Current Issue : July - September Volume : 2019 Issue Number : 3 Articles : 5 Articles
Screening of fetomaternal hemorrhage (FMH) is essential in management of fetomaternal antigen incompatibilities of blood.\nThe objective in this study was to evaluate the ability of automatic blood analyzer (ABA) to screen FMH, also comparing this\nmethod with flow cytometry (FCM). The contents of fetal red blood cells and fetal hemoglobin were evaluated by FCM and ABA,\nrespectively, using both blood samples of male adults laced with umbilical cord blood diluted at 1/10, 1/100, 1/1,000, and 1/10,000,\nor blood from puerperal women collected within 48 hours following delivery. FCM had better performance (area under curve,\nAUC = 0.8723) than ABA (AUC = 0.6569) in detecting fetal blood laced with blood from male adults. At a critical level of 0.5%,\nABA indicated that 27.5% of puerperal women would have FMH while FCM did not detect FMH. Our results showed that ABA\noverestimates FMHand disagrees with FCMon indicating puerperalwomen with FMH.ABAis inadequate for being used to screen\nfor or to measure FMH....
The purpose of an experimental design is to improve the productivity of experimentation. It is an efficient procedure for planning\nexperiments, so the data obtained can be analyzed to yield a valid and objective conclusion. This approach has been used as an\nimportant tool in the optimization of different analytical approaches. A D-optimal experimental design was used here, for the\nfirst time, to optimize the experimental conditions for the detection of reactive oxygen species (ROS) produced by human blood\nfrom healthy donors, a biological matrix that better resembles the physiologic environment, following stimulation by a potent\ninflammatory mediator, phorbol-12-myristate-13-acetate (PMA). For that purpose, different fluorescent probes were used,\nas�����.....
Myrrh essential oil (MEO) is widely used as remedies for the different human ailment in different parts of the world. Themisuse of\nthis natural product in higher doses may lead to fever, inflammation, and liver and kidney problems. In this study, we performed\nthe acute and subacute toxicity analysis ofMEO inmice model after subcutaneous injection and evaluated the safe dose to prevent\nthe possible risk and side effects. Initially (first phase study) higher dose of MEO..................
The effect of taxifolin on cisplatin-induced oxidative pulmonary damage was investigated biochemically and histopathologically in\nmale albino Wistar rats. There were four groups, with six animals in each group: 50 mg/kg of taxifolin plus 2.5 mg/kg of cisplatin\n(TC) group, 2.5 mg/kg of cisplatin only (CIS) group, 50 mg/kg of taxifolin only (TG) group, and a healthy control group (HG). In\nterms of the experimental procedure, the animals in the TC and TG groups were first treated via oral gavage. The CIS and HG\ngroups received distilled water as solvent, respectively. One hour later, the TC and CIS groups received cisplatin at a dose of\n2.5 mg/kg (injected intraperitoneally). Taxifolin, cisplatin, and the distilled water were administered at the indicated dose and\nvolume, using the same method daily for 14 d. At the end of this period, the animals were killed with a high dosage of\nthiopental anaesthesia (50 mg/kg). Blood and lung tissue samples were taken for biochemical (malondialdehyde (MDA),\nmyeloperoxidase (MPO), total glutathione (tGSH), and 8-hydroxy-2 deoxyguanosine (8-OHdG)) analyses and histopathological\nexaminations. The biochemical and histopathological results in the TC and HG groups were then compared with those in the\nCIS group. Cisplatin increased the levels of MDA, myeloperoxidase, and 8-OHdG, a marker of oxidative DNA damage, and\nreduced the amount of tGSH in the lung tissue. Moreover, severe alveolar damage, including oedema and extensive alveolar\nseptal fibrosis, in addition to infiltration of polymorphic nuclear leucocytes and haemorrhagic foci, was observed in the CIS\ngroup. These histopathological findings demonstrate that taxifolin provides protection against pulmonary oxidative stress by\npreventing increases in oxidant parameters and decreases in antioxidants....
Purpose. We sought to examine the possibility of reducing the contrast medium dosage in dual-energy imaging using a salinemixed\ninjection with a virtualmonochromatic energymethod of dual-source computed tomography (CT). Methods. An X-ray CT\n(SOMATOMDefinition Flash: Siemens, Nurnberg, Germany) was employed.The mixing ratio of contrast medium and saline was\ngradually changed by 10%, followed by a mixed injection into a dynamic blood flow phantom (Nemoto Kyorindo, Japan) which is\na hemodynamic simulation phantomto obtain time-enhancement curves (TECs). Exactly 64 TECs were prepared for each mixing\nratio by changing the energy from40 to 75 keV for monoenergetic imaging. The relationship between the image standard deviation\n(SD) and the energy of the virtual monochromatic image was determined. Combinations of the mixing ratio and energy (keV),\nwhich can maintain high CT numbers and low image SDs for 3D imaging, were tested, and the reduction rate of the contrast\nmedium was calculated. Results. The TECs for the mixed injection method changed linearly with the dilution rates. The mixing\nratios were strongly correlated with the maximum CT number of the TEC (R^2 = 0.98). Contrast CT numbers and image SDs\nincreased by approximately 20% and 25%, respectively, as the energy decreased by 5 keV.The optimal conditions for reducing the\ncontrast medium dose were a mixing ratio of 6:4 and 55 keV of energy. Conclusion. The virtual monochromatic energy method\nreduced the contrast medium dosage by up to 40% for three-dimensional CT-angio (3DCTA) tests....
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